anti p mek (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc anti p mek
Anti P Mek, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 494 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p+mek/Phospho-c-Raf+(Ser338)+Rabbit+mAb/pm41903755-382-46-47
Average 96 stars, based on 494 article reviews

anti p mek - by Bioz Stars, 2026-10

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Article Title: Collagen-mediated pro-tumorigenic MAPK activation drives stromal-immune reprogramming in solid cancers
Article Snippet: The following primary antibodies were used: p-MEK (Cat. 9154, Cell Signaling Technology; 1:1000), MEK (Cat. A4868, Abclonal; 1:1000), p-ERK (Cat. 9102, Cell Signaling Technology; 1:1000), ERK (Cat. A4782, Abclonal; 1:1000), and Vinculin (Cat. 66305-1-Ig, ProteinTech; 1:5000).

Article Title: Homoisoflavanone Delays Colorectal Cancer Progression via DNA Damage‐Induced Mitochondrial Apoptosis and Parthanatos‐Like Cell Death
Article Snippet: Protein concentrations were determined by BCA assay (P0012, Beyotime).

Article Title: Elucidating the material basis and mechanism of Shegan Mahuang decoction in inhibiting influenza virus pneumonia based on UHPLC-HRMS, network pharmacology and experimental verification.
Article Snippet: Ethnopharmacological relevance: Shegan Mahuang Decoction (SMD), a traditional Chinese medicinal prescription outlined in “Jin Gui Yao Lue”.. For approximately two thousand years, it has been widely used to treat asthma and flu-like symptoms in China and Japan for around twenty centuries.. While the efficacy is confirmed, its pharmacological mechanism remains unclear.

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Article Title: Adenylyl Cyclase 8 in Dorsal CA1 Neurons Prevents Depressive-Like Behaviors by Maintaining Neuronal Excitability and Glutamatergic Neurotransmission Through TIP39-PTH2R Signaling.
Article Snippet: .. Primary antibodies as follows: PKA (Cell signaling, 4782, 1:1000), p-PKA (Cell signaling, 5661, 1:500), MEK (Cell signaling, 8727, 1:1000), p-MEK (Cell signaling, 9154, 1:1000), ERK1/2 (Cell signaling, 4695, 1:1000) and p-ERK1/2 (Cell signaling, 4370, 1:1000), PTH2R (Proteintech, 14166-1-AP, 1:1000) and β- actin (Bioss, bsm-33036 m, 1:500) were used to incubate NC membrane overnight at 4 °C. .. Subsequently, membranes were washed and incubated for 1 h at room temperature with secondary antibodies which were used for recognizing the primary antibody, and ECL (Beyotime) was used for visualizing the target proteins.

Incubation:

Article Title: GAB2 regulates lipid metabolism by activating the MEK/ERK/c-Myc pathway: impact on renal cell carcinoma progression.
Article Snippet: Protein samples were separated through electrophoresis and transferred to PVDF membranes (Beyotime, FFP24). .. After incubation with 5% skim milk (Beyotime, P0216), membranes were incubated with primary antibodies including GAB2 (1:1000; Abcam, Cambridge, MA, USA; ab235932), MEK (1:1000; CST, Danvers, MA,USA; 9122), p-MEK (1:1000; CST, 9154), ERK (1:1000; CST, 9102), p-ERK (1:2000; CST, 4370), c-Myc (1:1000; CST, 9402), and GAPDH (1:10000; Abcam, ab181602) overnight at 4°C. .. Subsequently, membranes were treated with the secondary antibody (1:2000; Abcam, ab205718).

Article Title: GAB2 regulates lipid metabolism by activating the MEK/ERK/c-Myc pathway: impact on renal cell carcinoma progression
Article Snippet: Protein samples were separated through electrophoresis and transferred to PVDF membranes (Beyotime, FFP24). .. After incubation with 5% skim milk (Beyotime, P0216), membranes were incubated with primary antibodies including GAB2 (1:1000; Abcam, Cambridge, MA, USA; ab235932), MEK (1:1000; CST, Danvers, MA,USA; 9122), p-MEK (1:1000; CST, 9154), ERK (1:1000; CST, 9102), p-ERK (1:2000; CST, 4370), c-Myc (1:1000; CST, 9402), and GAPDH (1:10000; Abcam, ab181602) overnight at 4 °C. .. Subsequently, membranes were treated with the secondary antibody (1:2000; Abcam, ab205718) for 60 min.

Article Title: Homoisoflavanone Delays Colorectal Cancer Progression via DNA Damage-Induced Mitochondrial Apoptosis and Parthanatos-Like Cell Death.
Article Snippet: Samples were analyzed using a CytoFLEX S flow cytometer (Beckman Coulter), and data were processed using FlowJo. .. The membranes were then blocked with a 5% non-fat milk TBST (TBS containing 0.1% Tween-20) for 1 h at room temperature and incubated at 4◦C for overnight with primary antibodies against the following proteins: cleaved Caspase 3 (25128-1-AP, Proteintech), cleaved Caspase 8 (9496, CST), PARP (9542, CST), cleaved PARP (9541, CST), P21 (2947, CST), P53 (2527, CST), Cyclin A2 (91500, CST), Cyclin D1 (2978, CST), CDK1 (bs-1341R, Bioss), CDK2 (bs-10726R, Bioss), CDK7 (bs-0569R, Bioss), AKT (4691, CST), p-AKT (4060, CST), ERK (9102, CST), p-ERK (9101, CST), MEK (9126, CST), p-MEK (9154, CST), ATR (13934, CST), p-ATR (2853, CST), Chk1 (2360, CST), p-Chk1 (2348, CST), RAD51 (14961-1-AP, Proteintech), ATM (27156-1-AP, Proteintech), p-ATM (5883, CST), γ-H2AX (9718, CST), AIF (5318, CST), MIF (75038, CST), Cyt c (10993-1-AP, Proteintech), Lamin B (66095-1-Ig, Proteintech), VDAC1 (81538-1-RR, Proteintech) and GAPDH (60004-1-Ig, Proteintech). .. The cells were washed with PBS and stained with propidium iodide (F10797, Invitrogen) for 1 h at 4◦C in the dark.

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